Testing to see if 24h salinity ramps to 46psu increase survivorship post polyp bailout
date created 20210803; date last revised 20210803 hgr post creation
Overview
purpose: prevent cell lysis and micropropagule disentegration post polyp bailout. Previous bailout trials ramped Pocillopora acuta to 55-60psu. Most “surving (remaining)” micropropagule tissue did not have the morphological characteristics of recovered polyps and appeared as blobs and stains on settlement substrate photos in polyp bailout repo here. Though ramping to ~58-60psu is beneficial because it results in ~100% polyp bailout within 1d, we want to try ramps that will lessen salinity stress and hopefully promote survivorship and prevalence of “morpholigically” recovered micropropagules
objective: try and replicate figure 1 from Chuang & Mitarai 2020 Coral reefs. Chuang & Mitarai 2020 use a 24h ramp to increase salinity from 35psu to 46psu. info below
| comparison | our study | Chuang & Mitarai |
|---|---|---|
| total vol of tank | 2L | 5L |
| starting salinity | 35.5psu | 35ppt |
| ending salinity | psu | 46ppt |
| tank starting vol | 1.1L | ? |
| tank ending vol | 2L | ? |
| dosing schedule | 1 dos 96ml/hr; 4 dos 24ml/hr* | adding 4mL/min of 48ppt sw for 1d |
-* salinity of seawater being dosed in = 48.2psu -dosing started 20210803 4pm
corals used
| frag ID | tag ID | dosing treatment |
|---|---|---|
| s1 | 0001 | 1 dos/hr |
| s2 | 0001 | 1 dos/hr |
| s3 | 0001 | 1 dos/hr |
| s4 | 0001 | 1 dos/hr |
| s5 | 0026 | 4 dos/hr |
| s6 | 0026 | 4 dos/hr |
| s7 | 0026 | 4 dos/hr |
| s8 | 0026 | 4 dos/hr |
| a1 | 0031 | 1 dos/hr |
| a2 | 0031 | 1 dos/hr |
| a3 | 0031 | 1 dos/hr |
| a4 | 0031 | 1 dos/hr |
plan: take photos of fragments and symbiont cells each day. Salinity probe will track salinity every 10 min in the 1dos/hr container
added 2-3 ~0.5cm2 P. acuta fragments to each chamber (so samples could be sacrificed for symbiont sizes during ramping period)… frags in chambers here
